No

Usually translated “not”, “certainly” and “surely”. Open the dictionary entry below for the full range of meaning, with the verses it cites.

• not 1382
• certainly 95
• surely 70
• no longer 49
• no 42
• nothing 13
• neither 10
• none 8

Word-by-word English from the STEPBible tagged text.

• John 260
• Matthew 175
• Luke 159
• Mark 116
• 1 Corinthians 114
• Acts 105

27 books in all.

• 2 Thessalonians 2:10In contextAnd with all deceivableness of unrighteousness in them that perish; because they received not the love of the truth, that they might be saved.
• 2 Thessalonians 3:2In contextAnd that we may be delivered from unreasonable and wicked men: for all men have not faith.
• 2 Thessalonians 3:7In contextFor yourselves know how ye ought to follow us: for we behaved not ourselves disorderly among you;
• 2 Thessalonians 3:9In contextNot because we have not power, but to make ourselves an ensample unto you to follow us.
• 2 Thessalonians 3:10In contextFor even when we were with you, this we commanded you, that if any would not work, neither should he eat.
• 2 Thessalonians 3:14In contextAnd if any man obey not our word by this epistle, note that man, and have no company with him, that he may be ashamed.

From STEPBible’s Greek lexicon (based on Abbott-Smith).

How the English word “no” was understood when the King James Version was still the common Bible of English readers.

Noah Webster, An American Dictionary of the English Language (1828). Verse references are checked against the KJV. Read the full entry.